• July 25, 2024

Tissue Culture Technique Types

Using the technique of tissue culture, one may separate healthy tissues from live things or creatures. Depending on the technique, this might be the leaves or other plant components in plant tissue culture.

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Tissue culture is divided into the following categories according to the explant (plant tissue or beginning material) used to produce plants:

The Callus Culture

A callus is a group of undifferentiated cells that have the amazing capacity to develop into diverse plant components. Any plant organ’s derived plant tissues can create callus when intentionally induced in a lab environment. This callus then develops into various plant organs, roots, and shoots.

The Seed Culture

A common method of tissue culture used to cultivate a variety of plants, including orchids, in a laboratory setting is seed culture, which uses seeds as an explant.

The Protoplast Culture

A plant cell without a cell wall is called a protoplast. This method uses mechanical or enzymatic methods to remove the cell wall from plant cells. After being purified, the resultant protoplasts undergo controlled cell wall regeneration before being placed in the proper medium to continue growing into a whole plant.


In meristem culture, the meristematic area of a plant, such as the tips of its shoots, is removed and placed in a growth media that contains nutrients, vitamins, and hormones that are specific to plants. In the cultured cells, this method encourages tissue development and cell division. There are several uses for meristem culture, including as creating disease-free plants, regenerating whole plants, creating haploid and transgenic plants, improving crops, and conserving germplasm.

The Embryo Culture

Isolating and growing either immature or mature plant embryos in order to encourage their growth into full-grown plants is known as embryo culture. This approach entails sterilizing the organ (such as an ovule, seed, or fruit) from which the embryos are formed and using it in the culture process, as opposed to sterilizing each embryo separately.


The process entails cultivating plant species’ fertilized or unfertilized ovaries in an appropriate setting to speed up their growth into full plants. Gynogenesis, another name for this process, is mostly used to get through obstacles both before and after conception. It has also been used to produce interspecific hybrids.

Pollen/anther culture:

The male reproductive portions of flowers, known as pollen grains or anthers, are separated and cultivated in a nutrient-rich media using the pollen/anther cultivation technique in plant biotechnology. Using cultivated pollen or anther cells, this technique enables the growth and regeneration of haploid plants or callus tissues. In order to create novel plant types or investigate the behavior of plant cells in a controlled setting, it is frequently utilized in genetic and plant breeding research.

Why Is Tissue Culture Such a Great Idea?

Tissue culture has numerous useful uses and may be used to reproduce a wide range of species.

A plant’s yield may be quickly and significantly improved by employing the tissue culture method. Additionally, the plant may have its genetic makeup changed to make it resistant to specific infections and illnesses. Growers can guarantee that plants possess precise traits thanks to genetic manipulation. Businesses and people sometimes cultivate plants to have particular qualities that are more profitable for them or more appealing for their own usage.

Furthermore, a rare plant or endangered species may benefit from the tissue culture method of ensuring their existence.

Finally, the tissue culture method depends on the plant’s natural capacity to rapidly regenerate its cells, and these regenerated cells are copies that are most frequently referred to as clones. This method may be easily modified for a do-it-yourself project at home, or it can be utilized in a lab with pricey and complex equipment.